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CBP Faculty - Sanford H. Leuba, Ph.D.

Sanford H. Leuba, Ph.D.
Associate Professor
Tel: 412-623-7788
Fax: 412-623-4840
leuba@pitt.edu
PubMed pub. listing
Leuba Lab Website
CBP Research Group(s):
Regulation of Intracellular Signaling and Gene Expression
CBMP Graduate Program Groups:
DNA Damage/Repair, Cell-Cycle Control and Gene Expression, Cancer

Research Interest
Publications
  1. Rodriguez-Collazo P, Leuba SH, Zlatanova J. Robust methods for purification of histones from cultured mammalian cells with the preservation of their native modifications.Nucleic Acids Res. 2009 37:e81. Epub 2009 May 13.PMID: 19443446
  2. Anand, S. P., Zheng, H., Bianco, P., Leuba, S. H. and Khan, S. A. DNA helicase activity of PcrA is not required for the displacement of RecA from DNA and inhibition of RecA-mediated strand exchange. J. Bacteriology 189, 4502-4509, 2007.
  3. P. Rodriguez-Collazo, S. K. Snyder, R. C. Chiffer, J. Zlatanova, S. H. Leuba and C. L. Smith. cAMP Signaling Induces Rapid Loss of Histone H3 Phosphorylation in Mammary Adenocarcinoma-Derived Cell Lines. Experimental Cell Research. Epub ahead of print, 2007.
  4. Zheng, H., Goldner, L. S. and Leuba, S. H. Homebuilt scanning confocal fluorescence microscope for the study of DNA/protein interactions, one-at-a-time. Methods 41, 342-352, 2007.
  5. Zlatanova, J., William T. McAllister, W. T., Borukhov, S. and Leuba, S. H. Single-molecule approaches reveal the idiosyncrasies of RNA polymerases. Structure 14, 953-966, 2006.
  6. Tomschik, M., Zheng, H., van Holde, K., Zlatanova, J. and Leuba, S. H. (2005) Fast, long-range, reversible conformational fluctuations in nucleosomes revealed by spFRET. Proc. Natl. Acad. Sci. USA 102, 3278-3283.
  7. Zlatanova, J. & Leuba, S. H., Eds. (2004) Chromatin Structure & Dynamics: State-of-the-Art. Elsevier New Comprehensive Biochemistry, vol. 39, Amsterdam, 507 pg. ISBN: 0-444-515941.
  8. Leuba, S. H., Karymov, M. A., Tomschik, M., Ramjit, R., Smith, P. and Zlatanova, J. (2003) Assembly of single chromatin fibers depends on the tension in the DNA molecule: magnetic tweezers study. Proc. Natl. Acad. Sci. USA 100, 495-500.
  9. An, W., Palhan, V. B., Karymov, M. A., Leuba, S. H. and Roeder, R. G. (2002) Selective requirements for histone H3 and H4 N termini in p300-dependent transcriptional activation from chromatin. Mol. Cell 9, 811-821.
  10. Leuba, S. H. and Zlatanova, J., Eds. (2001) Biology at the single-molecule level. Pergamon, Amsterdam. ISBN: 0-08-044031-2 (Book reviewed by Ishii, Y. and Yanagida, T. Toward single molecule biochemistry. Cell 109, 686-688, 2002).
  11. Bennink, M. L., Leuba, S. H., Leno, G. H., Zlatanova, J., de Grooth, B. G. and Greve J. (2001) Unfolding individual nucleosomes by stretching single chromatin fibers with optical tweezers. Nat. Struct. Biol. 8, 606-610. Leuba, S. H. and Bustamante, C. (1999) Analysis of chromatin by scanning force microscopy. Methods Mol. Biol. 119, 143-160.
  12. Leuba, S. H., Bustamante, C., van Holde, K. and Zlatanova, J. (1998) Linker histone tails and N-tails of histone H3 are redundant: scanning force microscopy studies of reconstituted fibers. Biophys J. 74, 2830-2839.
  13. Zlatanova, J., Leuba, S. H. and van Holde, K. (1998) Chromatin fiber structure: morphology, molecular determinants, structural transitions. Biophys J. 74, 2554-2566.
  14. Leuba, S. H., Yang, G., Robert, C., Samori, B., van Holde, K., Zlatanova, J. and Bustamante, C. (1994) Three-dimensional structure of extended chromatin fibers as revealed by tapping-mode scanning force microscopy. Proc. Natl. Acad. Sci. USA 91, 11621-11625.