Syam P. Anand, Ph.D.

I am a Postdoctoral Research Associate in the laboratory of Professor Saleem Khan in the Department of Molecular Genetics and Biochemistry. With Saleem, I have shown that PcrA helicase is structure-specific and has both 3'?5' and 5'? helicase activities. As part of a collaborative study between the Khan and Leuba laboratories, I have been utilizing single-molecule approaches to study the mechanism of action of helicases. Haocheng and I are working closely, and combining both biochemical and single-molecule techniques to discover the precise activities of helicases.




	Syam P. Anand, Ph.D. B.Sc. Botany, Calicut University, India M.Sc., Microbiology, Mahatma Gandhi University, India Ph.D., Molecular Biology and Biochemistry, Indian Institute of Science, India

		I am a Postdoctoral Research Associate in the laboratory of Professor Saleem Khan in the Department of Molecular Genetics and Biochemistry. With Saleem, I have shown that PcrA helicase is structure-specific and has both 3'?5' and 5'? helicase activities. As part of a collaborative study between the Khan and Leuba laboratories, I have been utilizing single-molecule approaches to study the mechanism of action of helicases. Haocheng and I are working closely, and combining both biochemical and single-molecule techniques to discover the precise activities of helicases.


Selected Publications
Anilkumar, G., Srinivasan, R., Anand, S. P. and Ajitkumar, P. Bacterial cell division protein FtsZ is a specific substrate for the AAA family protease FtsH. Microbiology 147, 516-517 (2001). Chang, T. L., Naqvi, A., Anand, S. P., Kramer, M. G., Munshi, R. and Khan, S. A. Biochemical characterization of the staphylococcus aureus PcrA helicase and its role in plasmid rolling-circle replication. J. Biol. Chem. 277, 45880-45886 (2002). Anand, S. P., Mitra, P., Naqvi, A. and Khan, S. A. Bacillus anthracis and Bacillus cereus PcrA helicases can support DNA unwinding and in vitro rolling-circle replication of plasmid pT181 of Staphylococcus aureus. J. Bacteriol. 186, 2195-2199 (2004). Anand, S. P., Rajeswari, H., Gupta, P., Srinivasan, R., Indi, S. and Ajitkumar, P. A C-terminal deletion mutant of Mycobacterium tuberculosis FtsZ shows fast polymerization in vitro. Microbiology 150, 1119-1121 (2004). Anand, S. P. and Khan, S. A. Structure-specific DNA binding and bipolar helicase activities of PcrA. Nucleic Acids Res. 32, 3190-3197 (2004). Anand, S. P., Chattopadhyay, A. and Khan, S. A. The PcrA3 mutant binds DNA and interacts with the RepC initiator protein but is defective in its DNA helicase and unwinding activities. Plasmid 54, 104-113 (2005).